Cryoelectron microscopy of biological molecules is among the hottest growth areas in biophysics and structural
biology at present, and Frank is arguably the most distinguished practitioner of this art. CryoEM is likely over
the next few years to take over much of the structural approaches currently requiring X-ray crystallography, because
one can now get good and finely detailed images of single molecules down to as little as 200,000 MW, covering a
substantial share of the molecules of greatest biomedical research interest. This book, the successor to an earlier
work published in 1996 with Academic Press, is a natural companion work to our forthcoming book on electron crystallography
by Robert Glaeser, with contributions by six others, including Frank. A growing number of workers will employ CryoEM
for structural studies in their own research, and a large proportion of biomedical researchers will have a growing
interest in understanding what the capabilities and limits of this approach are.