"This book has a number of strengths, including the author's clear writing style and the large number of
informative two-color line drawings which make the book easy to read."
-- Choice, 10/99
Submitted by Publisher, August, 2001
Preface
The fields of biochemistry, genetics, and cell biology have been dramatically altered in the past two decades
by the development of an experimental approach that has been called recombinant DNA technology. In the past five
years, this laboratory methodology has expanded into a much wider domain of scientific investigation I have chosen
to call applied molecular genetics. One of the difficulties students have with applied molecular genetic
concepts is understanding how various molecular genetic techniques are combined to accomplish a research
goal. For many students, a conceptual gap exists between the methodological details found in laboratory protocol
books and the factual statements contained in biological textbooks. Undergraduate life science students often begin
their first hands-on research experience with only a vague idea of what "gene cloning" is all about,
and they usually lack a clear understanding of how to design an experimental strategy. Applied Molecular Genetics
attempts to fill this knowledge gap by presenting key biochemical and cell biological principles using simple
descriptive terms and illustrated flow schemes.
The organization of the book was chosen to facilitate its use as a resource for life science students and researchers
who need to see the forest among the trees. Toward this end, Applied Molecular Genetics is divided into
three sections to reflect the level of expertise required by the reader. Section 1 is called Laboratory Techniques
and it includes Chapters 1- 3. The topics presented in these chapters provide a "nuts and bolts" overview
of fundamental concepts in recombinant DNA technology. The material in Section 1 is best suited for readers who
have little or no experience performing nucleic acid biochemistry or gene cloning. Section 2, Core Methods, includes
Chapters 4 -6 and it represents the heart of applied molecular genetic concepts. This material systematically describes
the primary approaches and research objectives that characterize three major areas of applied molecular genetics:
manipulation of genomic DNA, isolation and characterization of gene coding sequences, and the polymerase chain
reaction. Section 2 is a good starting point for readers who have a basic under-standing of molecular genetic methods
but lack a clear idea of how various experimental tools are best applied to the study of complex biological problems.
Section 3, entitled Specialized Applications, comprises Chapters 7- 9. Though it would be expected that most life
science laboratory researchers would be interested in the core methodologies described in Sections 1 and 2, classroom
discussions may benefit more from material in Section 3 which describes cell culture models, animal transgenesis
and medical molecular genetics.
Each chapter concludes with a laboratory practicum describing the sequence of steps most often used in the
laboratory to implement a specific experimental objective. These laboratory practicums are similar in style to
the presentation of medical case studies in that the problem is first described, and then with text and illustrations,
a hypothetical flow scheme is presented. An extensive bibliography is also contained in every chapter to guide
readers both to landmark publications and to the most current literature in the field of applied molecular genetics.
In addition there are seven appendices (standard abbreviations, properties of nucleic acids, amino acids, restriction
enzymes, description of genetic markers, gel electrophoresis information, and URL addresses of useful Internet
sites) in Section 4 to provide laboratory researchers with essential biochemical information.
The descriptive style of the text makes it an ideal companion book for upper division undergraduate, graduate,
and medical school courses utilizing one of the larger molecular biology, biochemistry, or cell biology texts,
in which applied molecular genetic concepts are relegated to "shaded boxes" that contain only cursory
descriptions of essential techniques. Applied Molecular Genetics could also be used as a stand-alone text
for elective/ special topics courses typically offered to advanced life science students with some prior course
work in biochemistry, molecular biology, or cell biology. In this context, the many examples of molecular genetic
strategies, extensive primary references, and carefully chosen appendix materials provide the instructor with materials
to teach students how to design complex molecular genetic strategies using lab exercises and Internet resources.
Summary
Applied Molecular Genetics explains the key biochemical and cell biological principles behind some of today's
most commonly used applications of molecular genetics, using clear terms and well- illustrated flow schemes. The
book is divided into several sections and moves from basic to advanced topics while providing a concise overview
of fundamental concepts in modern biotechnology. Each chapter concludes with a Laboratory Practicum describing
a hypothetical research objective and the sequence of steps that are most often used to investigate biological
questions using molecular genetic methods. In addition, the book provides informative summaries of the latest advances
in molecular genetics, using attractive illustrations and a comprehensive reference list. Representative topics
include: 1) Genomic mapping using single nucleotide polymorphisms. 2) Creating cell-specific gene knockouts in
transgenic mice. 3) Animal cloning methodologies based on whole nuclear transfer. 4) Pharmacogenetic applications
of DNA microarray technology.
This text introduces the use of Internet resources through the World Wide Web as a powerful new tool in molecular
genetic research. Seven appendices are included in the book, providing a convenient resource for properties of
nucleic acids, protein and restriction enzymes, a description of common E. coli genetic markers and gel electrophoresis
parameters, as well as a list of useful Internet sites.
A must-have guide for all instructors and students in the fields of modern biochemistry, cell biology, and genetics,
Applied Molecular Genetics is an ideal reference for anyone needing a clear, concise source of up-to-date information
on this rapidly changing field.
Table of Contents
LABORATORY TECHNIQUES.
Biochemical Basis of Applied Molecular Genetics.
Laboratory Tools for Molecular Genetic Applications.
Overview of Gene Analysis Methods.
CORE METHODS.
Characterization of Genomic DNA.
Isolation and Characterization of Gene Transcripts.
The Polymerase Chain Reaction.
SPECIALIZED APPLICATIONS.
Expression of Cloned Genes in Cultured Cells.
Construction of Transgenic Multicellular Organisms.
Contemporary Applied Molecular Genetics.