"...Freshney provides 28 chapters that reflect the latest progress in media, specialized techniques, biotechnology,
DNA transfer and tumor culture."
--SciTech Book News,March 2001
Submitted by the Publisher, September, 2001
Summary
This book is a vital training manual of the fundamental principles and techniques of animal cell culture. Crucial
updates have been made in this edition to include advances in the fields of biology, media, specialized techniques,
biotechnology, DNA transfer, tumor culture, references, and suppliers.
Table of Contents
Biology of Cultured Cells.
Design and Layout.
Equipment.
Aseptic Technique.
Safety.
Culture Vessels.
Media.
Serum-Free Media.
Preparation and Sterilization.
Primary Culture.
Cell Lines.
Cloning and Selection.
Cell Separation.
Characterization.
Differentiation.
Transformation.
Cont amination.
Cryopreservation.
Quantitation.
Cytotoxicity.
Specialize d Cells.
Tumor Cells.
Organotypic Culture.
Scale -Up.
Specialized Techniques.
Molecular Techniques.
Problems-Solving.
In Conclusion.
