David H. Micklos and Greg A. Freyer. 1990. 477 pages. 244 illustrations. Combines the theory, practice, and
applications of recombinant DNA technology into one articulated package. Extensively tested by thousands of high
school and college teachers and students in 25 states and Canada, the 10 laboratory experiments cover basic techniques
of gene isolation and analysis. The experiments use systematic repetition to build student confidence and mastery
of techniques. Extensive prelab notes at the beginning of each experiment explain how to schedule and prepare,
while flow charts and icons make the protocols easy to follow. The laboratory course is completely supported by
quality-assured Carolina products--from bulk reagents, to reusable reagent systems, to single-use kits--satisfying
a broad range of teaching applications. Truly a first course in recombinant DNA technology, the laboratory sequence
presupposes no prior experience on the part of the instructor or student. Structured to follow directly from an
introduction to principles of biology, the experiments are equally appropriate for the advanced high school student
and the beginning college student. ISBN 0-89278-411-3. Soft cover.
The most rigorously critiqued, tested, and evaluated lab experiments in molecular biology today.
Appropriate for different levels of education, including research and nonresearch colleges and universities,
junior colleges, community colleges, and advanced biology programs in high school.
Successfully integrated into college level introductory biology, general biology, genetics, microbiology, cell
biology, molecular genetics, and molecular biology courses.
10 laboratory exercises, culminating in the construction and cloning of a recombinant DNA molecule.
Strictly adheres to National Institutes of Health (NIH) guidelines and lab safety procedures.