The increasing integration between gene manipulation and genomics is embraced in this new book, Principles of
Gene Manipulation and Genomics, which brings together for the first time the subjects covered by the best-selling
books Principles of Gene Manipulation and Principles of Genome Analysis & Genomics.
Comprehensively revised, updated and rewritten to encompass within one volume, basic and advanced gene manipulation
techniques, genome analysis, genomics, transcriptomics, proteomics and metabolomics
Includes two new chapters on the applications of genomics
An essential reference for upper level undergraduate and graduate students of genetics, genomics, molecular
biology and recombinant DNA technology.
Table of Contents
Preface
1. Gene Manipulation in the Post-Genomics Era
Part I: Fundamental Techniques of Gene Manipulation:
2. Basic Techniques
3. Cutting and Joining DNA Molecules
4. Basic Biology of Plasmid and Phage Vectors
5. Cosmids, Phasmids and Other Advanced Vectors
6. Gene Cloning Strategies
7. Sequencing Genes and Short Stretches of DNA
8. Changing Genes: Site-directed Mutagenesis and Protein Engineering
9. Bioinformatics
Part II: Manipulating DNA in Microbes, Plants and Animals:
10. Cloning in Bacteria Other Than Escherichia coli
11. Cloning in Saccharomyces cerevisiaea and Other Fungi
12. Gene Transfer to Animal Cells
13. Genetic Manipulation of Animals
14. Gene Transfer to Plants
15. Advanced Transgenic Technology
Part III: Genome Analysis, Genomics and Beyond:
16. The Organization and Structure of Genomes
17. Mapping and Sequencing Genomes
18. Comparative Genomics
19. Large-Scale Mutagenesis and Interference
20. Analysis of the Transcriptome
21. Proteomics I-Expression Analysis and Characterization of Proteins
22. Proteomics II-Analysis of Protein Structures
23. Proteomics III-Protein Interactions
24. Metabolomics and Global Biochemical Networks
Part IV: Applications of Gene Manipulation and Genomics:
25. Applications of Genomics: Understanding the Basis of Polygenic Disorders and Identifying Quantitative Trait
Loci
26. Applications of Recombinant DNA Technology
