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Methods in Cell Biology : Flow Cytometry, Part A., Volume 41
Methods in Cell Biology : Flow Cytometry, Part A., Volume 41
Author: Wilson / Matsudaira / Darzynkiewicz / Robinson / Crissman (Eds.)
Edition/Copyright: 2ND 95
ISBN: 0-12-203051-6
Publisher: Academic Press, Inc.
Type: Paperback
Other Product Information
Author Bio
Review
Summary
Table of Contents
 
  Author Bio

Wilson, Leslie (Ed.) : University of California-Santa Barbara

Matsudaira, Paul (Ed.) : Whitehead Institute for Biomedical Research / Massachusetts Institute of Technology

Darzynkiewicz, Zbigniew (Ed.) : New York Medical College

Robinson, J. Paul (Ed.) : Purdue University

Crissman, Harry A. (Ed.) : Biomedical Sciences Division, Los Alamos National Laboratories, New Mexico

 
  Review

Praise for the Second Edition:

"The second edition of Flow Cytometry provides a comprehensive and detailed foundation in the application of this diverse and rapidly expanding technology. The variety and scope of topics covered in this two-volume set are too large to cover in detail. The second edition of Flow Cytometry provides the investigator with a concise compilation of new and updated applications and procedures, updated references and a directory of the principal contributors to the field. The additions and improvements to the second edition are evidence of the impact this technology will continue to have on biological and medical sciences for years to come."

-- American Scientist

Academic Press Web Site, May, 2000

 
  Summary

Flow Cytometry, Second Edition provides a complete and comprehensive two volume laboratory guide and reference for the use of the most current methods in flow cytometry sample preparation and analysis. These essential techniques are described in a step-by-step format, supplemented by explanatory sections and trouble-shooting tips. The methods are accessible to all researchers and students in biomedical science and biology who must use flow cytometry to separate and analyze cells.

  • Completely revised and greatly expanded since the publication of the First Edition in 1990
  • Unique comprehensive methodological coverage
  • In depth treatment of procedures, including a description of each procedure's:
  • Theoretical foundations
  • Critical aspects
  • Possible pitfalls
  • Written by authors with extensive experience who:
  • Developed or modified the techniques
  • Describe their experience with different instruments and applications to different cell systems
  • Are the Who's Who in Flow Cytometry
  • Methods cover cell death and cell cycle analyses
  • Practical, handbook-style presentation works in lab or classroom
  • Color plates illustrate techniques
 
  Table of Contents
  1. Dissociation of Intact Cells from Tumors and Normal Tissues. D.W. Visscher and J.D. Crissman.
  2. Assays of Cell Viability: Discrimination of Cells Dying by Apoptosis. Z. Darzynkiewicz, X. Li, and J. Gong.
  3. Cell Preparation for the Identification of Leukocytes. C.C. Stewart and S.J Stewart.
  4. Multiparameter Analysis of Leukocytes by Flow Cytometry. C.C. Stewart and S.J. Stewart.
  5. Immunophenotyping Using Fixed Cells. G.F. Babcock and S.M. Dawes.
  6. Simultaneous DNA Content and Cell-Surface Immunofluorescence Analysis. P.P.T. Brons, P.E.J. Van Erp, and A.H.M. Pennings.
  7. Flow Cytometry Crossmatching for Solid Organ Transplantation. R.A. Bray.
  8. Cell Membrane Potential Analysis. H.M. Shapiro.
  9. Measurement of Intracellular pH. M.J. Boyer and D.W. Hedley.
  10. Intracellular Ionized Calcium. C.H. June and P.S. Rabinovitch.
  11. Cellular Protein Content Measurements. H.A. Crissman and J.A. Steinkamp.
  12. Lysosomal Proton Pump Activity: Supravital Cell Staining with Acridine Orange Differentiates Leukocyte Subpopulations. F. Traganos and Z. Darzynkiewicz.
  13. Staining of DNA in Live and Fixed Cells. H.A. Crissman and G.T. Hirons.
  14. High-Resolution Analysis of Nuclear DNA Employing the Fluorochrome DAPI. F.J. Otto.
  15. Detergent and Proteolytic Enzyme-Based Techniques for Nuclear Isolation and DNA Content Analysis. L.L. Vindeløv and I.J. Christensen.
  16. DNA Analysis from Paraffin-Embedded Blocks. D.W. Hedley.
  17. Controls, Standards, and Histogram Interpretation in DNA Flow Cytometry. L.G. Dressler and L.C. Seamer.
  18. DNA Content Histogram and Cell-Cycle Analysis. P.S. Rabinovitch.
  19. Immunochemical Quantitation of Bromodeoxyuridine: Application to Cell-Cycle Kinetics. F. Dolbeare and J.R. Selden.
  20. Application and Detection of IdUrd and CldUrd as Two Independent Cell-Cycle Markers. J.A. Aten, J. Stap, R. Hoebe, and P.J.M. Bakker.
  21. Cell-Cycle Analysis Using Continuous Bromodeoxyuridine Labeling and Hoechst 33358 (Ethidium Bromide Bivariate Flow Cytometry. M. Poot, H. Hoehn, M. Kubbies, A. Grossmann, Y. Chen, and P.S. Rabinovitch.
  22. Detection of BrdUrd-Labeled Cells by Differential Fluorescence Analysis of DNA Fluorochromes: Pulse-Chase and Continuous Labeling Methods. H.A. Crissman, N. Oishi, and R. Habbersett.
  23. Analysis of Intracellular Proteins. K.D. Bauer and J.W. Jacobberger.
  24. "Washless" Procedures for Nuclear Antigen Detection. J.K. Larsen.
  25. Light Scatter of Isolated Cell Nuclei as a Parameter Discriminating the Cell-Cycle Subcompartments. W. Giaretti and M. Nüsse.
  26. Simultaneous Analysis of Cellular RNA and DNA Content. Z. Darzynkiewicz.
  27. Analysis of DNA Content and Cyclin Protein Expression in Studies of DNA Ploidy, Growth Fraction, Lymphocyte Stimulation, and the Cell-Cycle. Z. Darzynkiewicz, J. Gong, and F. Traganos.
  28. Oxidative Product Formation Analysis by Flow Cytometry. J.P. Robinson, W.O. Carter, and P.K. Narayanan.
  29. Flow Cytometric Determination of Cysteine and Serine Proteinase Activities in Living Cells with Rhodamine 110 Substrates. S. Klingel, G. Rothe, W. Kellermann, and G. Valet.
  30. Leucine Aminopeptidase Activity by Flow Cytometry. J.J. Turek and J.P. Robinson.
  31. Enzyme Kinetics. J.V. Watson and C. Dive.
  32. On-Line Flow Cytometry: A Versatile Method for Kinetic Measurements. K. Nooter, H. Herweijer, R.R. Jonker, and G.J. van den Engh.
  33. Acid-Induced Denaturation of DNA in Situ as a Probe of Chromatin Structure. Z. Darzynkiewicz.
  34. Detection of Intracellular Virus and Viral Products. J. Laffin and J.M. Lehman.



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